A completely filled ethyl alcohol container is heavy. Ask for assistance if container is too heavy to lift. Ethyl alcohol gives off strong fumes; wear a face mask to avoid dizziness. Also, wear goggles (to prevent ethyl alcohol from splashing in eyes) and gloves (to protect skin). Mix ethyl alcohol in fume hood.
15-20 minutes
| Step | Image |
| Gather all the necessary supplies. |
|
| The red ethyl alcohol container is located under the Thrips 3 computer in PSL 1030. This container should be labeled as 95% ethyl alcohol. |
|
| Pour the amount of ethyl alcohol that you think you will use into a widemouth beaker (see table below for estimates). Then, using a graduated cylinder, dispense the appropriate volume of ethyl alcohol into designated spray bottles. The appropriate percent concentration of ethyl alcohol is labeled on the bottles; refer to the table below for dilution measurements. |
|
| Add the proper amount of deionized water to the
ethyl alcohol in the bottles. |
|
| Be sure that the lids are screwed on
tightly. |
|
| If quite a bit of ethyl alcohol is left over in the beaker, find another spray bottle and make an extra solution to avoid waste. Extra 95% ethyl alcohol can be temporarily stored in a parafilm-covered flask with proper labeling; covered flasks should be placed in the fume hood. Small amounts of alcohol may be disposed of in lab sinks. |
|
Measurements for Dilution | ||
| concentration | deionized water (ml) | 95% ethyl alcohol (ml) |
|
50% |
225 |
275 |
|
75% |
100 |
400 |
|
95% |
0 |
500 |