Protocol Sheets

Safety

• Laboratory 
• Greenhouse

Thrips Colony

• Colony information
• Daily care
• Thrips materials
• Bean washing
• Cut flowers
• Bean seedling
• Honey
• Cleaning cages
• Washing thrips materials
• Bean mixing
• Rearing same aged thrips
• Greenhouse thrips care

Plant Care

• Greenhouse sanitation
• Cross house sanitation
• Adding fertilizer to tanks in 2E5
• Cleaning ebb and flood benches
• Draining ebb and flood tanks
• Taking cuttings
• Seeding
• Transplanting
• Watering plants
• Soil testing

Insect Cages

• Preference cage setup and construction
• Preference cage cleaning
• Display cage construction
• Tube cage construction
• Washing tube cages
• Leaf cage construction
• Colony cage construction

Growth Chamber Maintenance

• Changing lamps
• Humidity sensor

General Lab Equipment

• Dataloggers
• Microscope camera
• Calibrating thermometers
• Drierite recharge

Insect Inoculation and Evaluation

• Inoculating tube cages
• Thrips extraction
• Inoculating single leaf cages
• Evaluating damage in single leaf cages

Computers

• Data backup
• Transfer of files to server
• Creating PDF files
• Scanning slides with flatbed scanner
• Thesis template
• Creating screen captures

Image Analysis

• Color correction of images
• Cropping images
• Area analysis
• Damage analysis
• Script modification

Autoclave Procedures

• Liquid and gravity runs
• Autoclaving hydrocorn (small autoclave) 
• Autoclaving hydrocorn (greenhouse autoclave)

Mixing Solutions

• Filling ethyl alcohol bottles  

Agar

• Making agar
• Agar disposal